Process development for expansion of human mesenchymal stromal cells in a 50L single-use stirred tank bioreactor
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چکیده
منابع مشابه
Expansion of mesenchymal adipose-tissue derived stem cells in a stirred single-use bioreactor under low-serum conditions
Materials and methods Preliminary tests in disposable-spinners (100 mL culture volume, Corning) were used to determine two suitable media and MC-types for serum reduced expansions (< 10%) of human adipose tissue-derived stem cells (hADSCs; passage 2, Lonza). Using such optimized media-MC-combinations, hADSCs expanded 30 to 40fold, which compares well with expansion rates in planar culture. Base...
متن کاملControlled expansion and differentiation of mesenchymal stem cells in a microcarrier based stirred bioreactor
Introduction Cell based therapy requires great numbers of cells in a functional state permitting their in vivo implantation for the restoration of tissue homeostasis. Three main parameters are believed to be essential for such a purpose: an appropriate cell population, a suitable scaffold and appropriate physical / biochemical factors enabling proper expansion and in vitro cell differentiation....
متن کاملModification and qualification of a stirred single-use bioreactor for the improved expansion of human mesenchymal stem cells at benchtop scale
Valentin Jossen*,1, Stephan C Kaiser1, Carmen Schirmaier1, Jacqueline Herrmann2, Alexander Tappe2, Dieter Eibl1, Ann Siehoff3, Christian van den Bos3 & Regine Eibl1 Zurich University of Applied Sciences, School of Life Sciences & Facility Management, Institute of Biotechnology, Grüental, 8820 Wädenswil, Switzerland Sartorius Stedim Biotech, August-Spindler-Str. 11, 37079 Göttingen, Germany Lonz...
متن کاملCharacterization and performance of the Allegro™ STR 200 single-use stirred tank bioreactor
Background Currently, stirred tank reactors (STR) represent the gold standard for the large scale growth of suspension cell lines. Culture performance is strongly influenced by the efficiency of mixing, as measured by the mass transfer coefficient and mixing time. The success of traditional stainless steel or glass STR systems lies in their impeller driven agitation that efficiently mixes large...
متن کاملCharacterization and performance of the Allegro™ STR 1000 single-use stirred tank bioreactor system
Background The benefits of single-use technologies in both upstream and downstream operations are now widely acknowledged by the biopharmaceutical industry, and have resulted in radical changes to the design and operation of many pharmaceutical processes. In mammalian cell culture, multi-use, cleanable glass or stainless steel stirred tank reactors (STRs) have been used successfully for growth ...
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ژورنال
عنوان ژورنال: Biochemical Engineering Journal
سال: 2017
ISSN: 1369-703X
DOI: 10.1016/j.bej.2016.11.020